中国水稻科学 ›› 2012, Vol. 26 ›› Issue (4): 417-422.DOI: 10.3969/j.issn.10017216.2012.04.005

• 研究报告 • 上一篇    下一篇

一个控制水稻株高QTL qPH3的遗传分析

罗炬#,邵高能#,魏祥进,陈明亮,唐绍清,焦桂爱,谢黎虹,胡培松   

  1. 中国水稻研究所 国家水稻改良中心/水稻生物学国家重点实验室, 浙江 杭州 310006;
  • 收稿日期:2011-07-20 修回日期:2011-10-08 出版日期:2012-07-10 发布日期:2012-07-10
  • 通讯作者: 胡培松*
  • 基金资助:

    国家973计划资助项目(2009AA101101); 国家自然科学基金资助项目(31000702); 浙江公益技术应用研究资助项目(2010C32G3010027); 国家转基因生物新品种培育科技重大专项(2011ZX08001006)。

Genetic Analysis of a QTL qPH3 for Plant Height in Rice

LUO Ju#, SHAO Gaoneng#, WEI Xiangjin, CHEN Mingliang, TANG Shaoqing, JIAO Guiai, XIE Lihong, HU Peisong*   

  1. China National Rice Research Institute,  National Center for Rice Improvement/State Key Laboratory of Rice Biology, Hangzhou 310006,  China;
  • Received:2011-07-20 Revised:2011-10-08 Online:2012-07-10 Published:2012-07-10
  • Contact: HU Peisong*

摘要: 利用D50/HB277衍生的重组自交系群体,对水稻株高进行QTL分析。在杭州和海南两种环境条件下,共检测到6个控制株高的加性效应QTL,分别位于第1、2、3、4、6和8染色体上,其中,位于第3染色体的QTL qPH3效应较大,在两种环境条件均能稳定表达且贡献率分别为16.52%和16.60%。进一步筛选获得1个剩余杂合体,对其衍生的RHLF2群体进行遗传分析,其qPH3检测贡献率达76.13%。通过后代验证,最终将其锁定于标记RM16211和RM16237之间物理距离约204 kb区域内。这将为该基因的精细定位及克隆奠定基础。

关键词: 水稻, 重组自交系, 株高, 数量性状座位, 剩余杂合体

Abstract: A recombinant inbred population derived from the  cross between  a javonica(D50)and an indica(HB277)rice was used to investigate the genetic factors affecting plant height. A total of six quantitative trait loci(QTLs)with significant additive effect were identified for the plant height, and they were located on chromosome 1, 2, 3, 4, 6 and 8. One QTL qPH3 can stably express and have significant additive effects in Hangzhou, Zhejiang Province  with the explained variance of 16.52% and Linshui, Hainan  Province with the explained variance of 16.60%. One residual heterozygous line(RHL) was selected from the RILs  and a RHLF2 population was constructed. The    major QTL qPH3 with the explained variance of 76.13% was detected with the RHLF2 population. By progeny tests,  qPH3  was  finally mapped within about 204 kb in the region RM16211-RM16237. These results will be helpful for fine mapping and mapbased cloning of this QTL.

Key words: rice, recombinant inbred line, plant height, quantitative trait loci, residual heterozygous line

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